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Stable Cell Line Generation Services | VectorBuilder

(6 day ago) VectorBuilder can generate stable cell lines with permanent knockout of your GOI (s). Stable knockout of GOI (s) is achieved using a CRISPR-based approach, wherein a pair of gRNAs targeting the GOI (s) is introduced into the target cells along with Cas9. This leads to the generation of two cuts on the target gene.

https://en.vectorbuilder.com/products-services/service/stable-cell-line-generation.html

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Generation of Stable Expression Mammalian Cell Lines Using

(6 day ago) The technique of generating stable cell lines using 3 rd generation lentivirus is very robust and it typically takes about 1-2 weeks to get stable expression for most mammalian cell lines. The advantage of using the 3 rd generation lentivirus are that are very safe and they are replication incompetent.

https://pubmed.ncbi.nlm.nih.gov/30505888/

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Generation of a stable packaging cell line producing high

(27 day ago) to packaging cell lines. Stable transfection of vector DNA to packaging cells is the second methodology described in this study for generating stable IDLV producing cell lines. We employed this methodology (which is independent of the vector's integration efficiency) to incorporate PPT-deleted vectors into packaging cell lines.

https://www.cell.com/molecular-therapy-family/methods/fulltext/S2329-0501(16)30037-7

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Generation of stable PDX derived cell lines using

(7 day ago) Human lung and ovarian PDX tumors were successfully propagated using CR technology to create stable explant cell lines (CR-PDX). These CR-PDX cell lines maintained parental driver mutations and allele frequency without clonal drift.

https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-017-0745-1

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Generation of Stable Cell Line in 28 Days | Altogen Labs

(5 day ago) Altogen Labs offers several stable cell line generation services: protein overexpressing cell lines, RNAi knockdown cell lines, and reporter stable cell lines (luciferase, GFP, RFP, YFP). Company scientists provide expert services with years of cell culture experience with more than 150 cancer cell lines (available in-house).

http://altogenlabs.com/generation-of-stable-cell-lines/generation-of-stable-cell-line-in-28-days/

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What are the best possible ways to generate stable cell

(6 day ago) Lentivirus is really an efficient way to generate stable cell lines. Integration into host cell genome, it mediates long-term and stable expression of exogenous genes with a simple system for

https://www.researchgate.net/post/What-are-the-best-possible-ways-to-generate-stable-cell-lines-of-mammalian-cell-lines-like-HEK293-or-COS7

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Antibody expressing stable cell lines – Welgen Inc.

(9 day ago) Premium service of clonal stable cell line generation for antibody production includes basic service and the following: a. Subcloning the top 3 clones for a second round of selection including screening of up to 72 clones; b. Top three clones selected from the second round and characterized for viability, cell growth, and titer measurements

https://www.welgen.com/generation-of-stable-cell-line-expressing-antibodies/

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Stable Cell Line Generation - ALSTEM, LLC

(11 day ago) Stable Cell Line Generation Request Form. Customer Information. Project Information. Stable Cell Line: GOI overexpression Reporter Cell Line TET inducible cell line GFP RFP Luciferase Other Service Type: Cell pool

https://www.alstembio.com/web/services/stable-cell-gen-webform.php

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Anyone have experience with stable cell line generation?

(10 day ago) You may try ecopack-293 to produce the first virus and then use it to infect Pt67 to produce the virus you will use for stable cell line generation. After infection of Pt67, you may harvest the

https://www.researchgate.net/post/Anyone_have_experience_with_stable_cell_line_generation

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