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Generate Stable Cell Lines With Chemical Transfection

(5 day ago) Most cell biology experiments utilize transient transfection protocols that afford peak gene expression between 24-96 hours post transfection. However, if sustained gene expression is required for longer periods of time, generation of stable cell lines is a viable option.

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How to Make Stable Transfected Cell Line - Pediaa.Com

(3 day ago) Stable transfection is the long-term introduction of foreign DNA into cells. Stably transfected cells pass the foreign DNA through progeny. Therefore, to make stably transfected cell lines, foreign DNA has to be integrated into the genome of the cell line. However, stable inheritance can be observed in nongenomic DNA as well.

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Addgene: Virus Protocol - Generating Stable Cell Lines

(4 day ago) Introduction. This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector. Unlike the short term protein expression observed using transient transfection approaches, generating cell lines using lentiviral vectors enables long-term protein expression studies.

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What are the best possible ways to generate stable cell

(3 day ago) What are the best possible ways to generate stable cell lines of mammalian cell lines, like HEK293 or COS7? Also, for making stable cell line, I would use antibiotics selectionstarting to

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Stable Cell Line Protocol - University of San Diego

(5 day ago) The amount of G418 required to kill cells not expressing the construct will vary from cell line to cell line. We have titrated the amount required for our HeLa cell lines, and this can be a starting point for other cell lines. Initially we use 400 ug/ml G418 in the media when selecting for stable clones. Cell Line Protocol.pdf

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Protocol of Stable Cell Line Generation - Creative BioMart

(2 day ago) 4. Analysis of Stable Cell Lines. Once you have obtained resistant cell lines, you should expand the cells and assay your target gene compared with positive and negative control. You can detect the expression of fusion protein by an appropriate analysis method such as western blot, microscopy, ELISA, as well as flow cytometry.

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How can I make a stable HEK293 cell line using pcDNA3.1?

(12 day ago) I am working to make a stable cell line of Rabbit kidney 13 cells (RK13) expressing one of the herpes virus proteins, I have determined the killing dose of Geneticine, and I am going to transfect

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Stable Transfection | Thermo Fisher Scientific - US

(2 day ago) Puromycin, a translation inhibitor in both prokaryotic and eukaryotic cells, is an aminonucleoside antibiotic from Streptomyces alboniger.Resistance is conferred by the puromycin N-acetyltransferase gene (pac) from Streptomyces.Puromycin has a fast mode action, causing rapid cell death even at low antibiotic concentrations, allowing the generation of puromycin-resistant stable cell lines in

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